請輸入關鍵字
請輸入關鍵字
訂購
*國家
中國
美國
中國香港
中國澳門
中國台灣
阿爾巴尼亞
阿爾及利亞
阿根廷
阿拉伯聯合酋長國
阿魯巴
阿曼
阿塞拜疆
阿森鬆島
埃及
埃塞俄比亞
愛爾蘭
愛沙尼亞
安道爾
安哥拉
安圭拉
安提瓜和巴布達
奧地利
奧蘭群島
澳大利亞
巴巴多斯
巴布亞新幾內亞
巴哈馬
巴基斯坦
巴拉圭
巴勒斯坦領土
巴林
巴拿馬
巴西
白俄羅斯
百慕大
保加利亞
北馬裏亞納群島
貝寧
比利時
冰島
波多黎各
波蘭
波斯尼亞和黑塞哥維那
玻利維亞
伯利茲
博茨瓦納
不丹
布基納法索
布隆迪
朝鮮
赤道幾內亞
丹麥
德國
迪戈加西亞島
東帝汶
多哥
多米尼加共和國
多米尼克
俄羅斯
厄瓜多爾
厄立特裏亞
法國
法羅群島
法屬波利尼西亞
法屬圭亞那
法屬南部領地
梵蒂岡
菲律賓
斐濟
芬蘭
佛得角
福克蘭群島
岡比亞
剛果(布)
剛果(金)
哥倫比亞
哥斯達黎加
格恩西島
格林納達
格陵蘭
格魯吉亞
古巴
瓜德羅普
關島
圭亞那
哈薩克斯坦
海地
韓國
荷蘭
荷屬加勒比區
荷屬聖馬丁
黑山
洪都拉斯
基裏巴斯
吉布提
吉爾吉斯斯坦
幾內亞
幾內亞比紹
加拿大
加納
加納利群島
加蓬
柬埔寨
捷克
津巴布韋
喀麥隆
卡塔爾
開曼群島
科科斯(基林)群島
科摩羅
科索沃
科特迪瓦
科威特
克羅地亞
肯尼亞
庫克群島
庫拉索
拉脫維亞
萊索托
老撾
黎巴嫩
立陶宛
利比裏亞
利比亞
聯合國
列支敦士登
留尼汪
盧森堡
盧旺達
羅馬尼亞
馬達加斯加
馬恩島
馬爾代夫
馬耳他
馬拉維
馬來西亞
馬裏
馬其頓
馬紹爾群島
馬提尼克
馬約特
毛裏求斯
毛裏塔尼亞
美國本土外小島嶼
美屬薩摩亞
美屬維爾京群島
蒙古
蒙特塞拉特
孟加拉國
秘魯
密克羅尼西亞
緬甸
摩爾多瓦
摩洛哥
摩納哥
莫桑比克
墨西哥
納米比亞
南非
南極洲
南喬治亞和南桑威奇群島
南蘇丹
瑙魯
尼加拉瓜
尼泊爾
尼日爾
尼日利亞
紐埃
挪威
諾福克島
帕勞
皮特凱恩群島
葡萄牙
日本
瑞典
瑞士
薩爾瓦多
薩摩亞
塞爾維亞
塞拉利昂
塞內加爾
塞浦路斯
塞舌爾
沙特阿拉伯
聖巴泰勒米
聖誕島
聖多美和普林西比
聖赫勒拿
聖基茨和尼維斯
聖盧西亞
聖馬丁島
聖馬力諾
聖皮埃爾和密克隆群島
聖文森特和格林納丁斯
斯裏蘭卡
斯洛伐克
斯洛文尼亞
斯瓦爾巴和揚馬延
斯威士蘭
蘇丹
蘇裏南
所羅門群島
索馬裏
塔吉克斯坦
泰國
坦桑尼亞
湯加
特克斯和凱科斯群島
特裏斯坦-達庫尼亞群島
特立尼達和多巴哥
突尼斯
圖瓦盧
土耳其
土庫曼斯坦
托克勞
瓦利斯和富圖納
瓦努阿圖
危地馬拉
委內瑞拉
文萊
烏幹達
烏克蘭
烏拉圭
烏茲別克斯坦
希臘
西班牙
西撒哈拉
新加坡
新喀裏多尼亞
新西蘭
匈牙利
休達及梅利利亞
敘利亞
牙買加
亞美尼亞
也門
伊拉克
伊朗
以色列
意大利
印度
印度尼西亞
英國
英屬維爾京群島
英屬印度洋領地
約旦
越南
讚比亞
澤西島
乍得
直布羅陀
智利
中非共和國
*省份
*城市
*姓名
*電話
*單位
*職位
*郵箱
*請輸入驗證碼
*驗證碼
B-hCXCR2 mice
Strain Name
C57BL/6-Cxcr2tm1(CXCR2)Bcgen/Bcgen 
Common Name  B-hCXCR2 mice
Background C57BL/6 Catalog number  110816
Related Genes 
C-X-C motif chemokine receptor 2, CD182, CDw128b, CMKAR2, IL8R2, IL8RA, IL8RB

Gene description

Chemokines are a group of small, mostly basic molecules that regulate cell trafficking of various leukocytes through interactions with a subset of 7-transmembrane G protein-coupled receptors. Chemokines mainly act on neutrophils, monocytes, lymphocytes, and eosinophils and play a pivotal role in host defense mechanisms. CXCR2 is a promiscuous receptor for several CXCL chemokines, including CXCL1, CXCL2, CXCL3, CXCL5, CXCL6, CXCL7, and CXCL8. It binds to IL8 with high affinity, and transduces the signal through a G-protein activated second messenger system. This receptor also binds to chemokine (C-X-C motif) ligand 1 (CXCL1/MGSA), a protein with melanoma growth stimulating activity, and has been shown to be a major component required for serum-dependent melanoma cell growth. This receptor mediates neutrophil migration to sites of inflammation. The angiogenic effects of IL8 in intestinal microvascular endothelial cells are found to be mediated by this receptor. Knockout studies in mice suggested that this receptor controls the positioning of oligodendrocyte precursors in developing spinal cord by arresting their migration.


mRNA expression analysis


from clipboard


Strain specific analysis of CXCR2 gene expression in WT and B-hCXCR2 mice by RT-PCR. Mouse Cxcr2 mRNA was detectable in splenocytes of wild-type (+/+) mice. Human CXCR2 mRNA was detectable only in H/H, but not in +/+ mice. 


Protein expression analysis in Gr-1+ cells in spleen 



Strain specific CXCR2 expression analysis in heterozygous B-hCXCR2 mice by flow cytometry. Splenocytes were collected from WT and heterozygous B-hCXCR2 (H/H) mice, and analyzed by flow cytometry with species-specific CXCR2 antibody. Mouse CXCR2 was detectable in WT mice and heterozygous B-hCXCR2. Human CXCR2 was exclusively detectable in heterozygous B-hCXCR2 but not WT mice.


Protein expression analysis in Gr-1+ cells in bone marrow




Strain specific CXCR2 expression analysis in homozygous B-hCXCR2 mice by flow cytometry. Splenocytes were collected from WT and homozygous B-hCXCR2 (H/H) mice, and analyzed by flow cytometry with species-specific CXCR2 antibody. Mouse CXCR2 was detectable in WT mice. Human CXCR2 was exclusively detectable in homozygous B-hCXCR2 but not WT mice.

Analysis of T cell subpopulation in spleen

from clipboard

Analysis of spleen leukocyte subpopulations by FACS. Splenocytes were isolated from female C57BL/6 and B-hCXCR2 mice (n=3, 7-week-old). Flow cytometry analysis of the splenocytes was performed to assess leukocyte subpopulations. A. Representative FACS plots. Single live cells were gated for the CD45+ population and used for further analysis as indicated here. B. Results of FACS analysis. Percent of T cells, B cells, NK cells, dendritic cells, granulocytes, monocytes and macrophages in homozygous B-hCXCR2 mice were similar to those in the C57BL/6 mice, demonstrating that CXCR2 humanized does not change the overall development, differentiation or distribution of these cell types in spleen. Values are expressed as mean ± SEM.


Analysis of T cell subpopulation in spleen


from clipboard

Analysis of spleen T cell subpopulations by FACS. Splenocytes were isolated from female C57BL/6 and B-hCXCR2 mice (n=3, 7-week-old). Flow cytometry analysis of the splenocytes was performed to assess leukocyte subpopulations. A. Representative FACS plots. Single live CD45+ cells were gated for TCRβ+ T cell population and used for further analysis as indicated here. B. Results of FACS analysis. The percent of CD4+ T cells, CD8+ T cells and Tregs in homozygous B-hCXCR2 mice were similar to those in the C57BL/6 mice, demonstrating that introduction of hCXCR2 in place of its mouse counterpart does not change the overall development, differentiation or distribution of these T cell subtypes in spleen. Values are expressed as mean ± SEM.

Analysis of leukocytes cell subpopulation in lymph node(LNs)


from clipboard

Analysis of LNs leukocyte subpopulations by FACS. LNs were isolated from female C57BL/6 and B-hCXCR2 mice (n=3, 7-week-old). Flow cytometry analysis of the LNs was performed to assess leukocyte subpopulations. A. Representative FACS plots. Single live cells were gated for the CD45+ population and used for further analysis as indicated here. B. Results of FACS analysis. Percent of T cells, B cells and NK cells in homozygous B-hCXCR2 mice were similar to those in the C57BL/6 mice, demonstrating that CXCR2 humanized does not change the overall development, differentiation or distribution of these cell types in LNs. Values are expressed as mean ± SEM.


Analysis of T cell subpopulation in lymph node(LNs)


from clipboard

Analysis of LNs T cell subpopulations by FACS. LNs were isolated from female C57BL/6 and B-hCXCR2 mice (n=3, 7-week-old). Flow cytometry analysis of the LNs was performed to assess leukocyte subpopulations. A. Representative FACS plots. Single live CD45+ cells were gated for TCRβ+ T cell population and used for further analysis as indicated here. B. Results of FACS analysis. The percent of CD4+ T cells, CD8+ T cells and Tregs in homozygous B-hCXCR2 mice were similar to those in the C57BL/6 mice, demonstrating that introduction of hCXCR2 in place of its mouse counterpart does not change the overall development, differentiation or distribution of these T cell subtypes in LNs. Values are expressed as mean ± SEM.


Analysis of leukocytes cell subpopulation in blood


from clipboard

Analysis of blood leukocyte subpopulations by FACS. Blood were isolated from female C57BL/6 and B-hCXCR2 mice (n=3, 7-week-old). Flow cytometry analysis of the blood was performed to assess leukocyte subpopulations. A. Representative FACS plots. Single live cells were gated for the CD45+ population and used for further analysis as indicated here. B. Results of FACS analysis. Percent of T cells, B cells, NK cells, dendritic cells, granulocytes, monocytes and macrophages in homozygous B-hCXCR2 mice were similar to those in the C57BL/6 mice, demonstrating that CXCR2 humanized does not change the overall development, differentiation or distribution of these cell types in blood. Values are expressed as mean ± SEM.

Analysis of T cell subpopulation in blood

from clipboard

Analysis of blood T cell subpopulations by FACS. Blood were isolated from female C57BL/6 and B-hCXCR2 mice (n=3, 7-week-old). Flow cytometry analysis of the blood was performed to assess leukocyte subpopulations. A. Representative FACS plots. Single live CD45+ cells were gated for TCRβ+ T cell population and used for further analysis as indicated here. B. Results of FACS analysis. The percent of CD4+ T cells, CD8+ T cells and Tregs in homozygous B-hCXCR2 mice were similar to those in the C57BL/6 mice, demonstrating that introduction of hCXCR2 in place of its mouse counterpart does not change the overall development, differentiation or distribution of these T cell subtypes in blood. Values are expressed as mean ± SEM.

Efficacy Validation on IBD

from clipboard

B-hCXCR2 mice were provided drinking water containing DSS for 7 consecutive days, and body weight changes were recorded throughout and scored clinically. (Fig. A-C) The body weight changes of animals in each group. (Fig. D) DAI score (disease activity index) of animals in each group. Compared with the vehicle group (G1), the body weight in the model group (G2) was significantly decreased and the DAI score was significantly increased, which indicated that the disease severity in the model group was aggravated; On the contrary, the body weight and the DAI score were significantly improved in the treatment group (G3). The results demonstrated that the DSS-induced inflammatory bowel disease model in B-hCXCR2 mice can be established successfully, and anti-hCXCR2 antibody H3h9 (in house) relieved the clinical symptoms of IBD. Values are expressed as mean ± SEM.