mRNA expression analysis

Strain specific analysis of IL11RA gene expression in wild-type mice and B-hIL11RA mice by RT-PCR.Mouse Il11ra1 mRNA was detectable in liver of wild-type mice (+/+). Human IL11RA mRNA was detectable only in homozygous B-hIL11RA but not in wild-type mice.

Protein expression analysis

Strain specific IL11RA expression analysis in homozygous B-hIL11RA mice by western blot.Tissue was collected from wild-type C57BL/6 mice (+/+) and homozygous B-hIL11RA mice (H/H), and analyzed by western blot with anti-IL11RA antibody. Mouse IL11RA1 and human IL11RA were detectable in wild type mice and homozygous B-hIL11RA mice, as the antibody is crossly reactive with IL11RA in human and mice. Mice IL11RA1 and human IL11RA were not detectable in kidney, which might be because of low protein levels compared with liver.

In vivo efficacy of anti-human IL11RA antibody with APAP-induced liver damage in B-hIL11RA mice