B-hIL33/hTSLP/hTSLPR mice
| Strain Name | C57BL/6-Il33tm1(IL33)Tslptm1(TSLP)Crlf2tm2(CRLF2)/Bcgen | Common Name | B-hIL33/hTSLP/hTSLPR mice |
| Background | C57BL/6 | Catalog number | 111867 |
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Related Genes |
IIL33: C9orf26, DVS27, IL1F11, NF-HEV, NFEHEV TSLP: NA;CRLF2: CRL2Y, TSLPR |
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Strain specific IL33 and TSLP expression analysis in homozygous B-hIL33/hTSLP/hTSLPR mice by ELISA. Calcipotriol (MC903) was dissolved in ethanol and topically applied on ears of either wild type C57BL/6 mice (+/+) or homozygous B-hIL33/hTSLP/hTSLPR mice (H/H) for 7 days. n=3. Ear grinding supernatant from the two mice was analyzed by ELISA. Mouse IL33 and TSLP were only detectable in wild type C57BL/6 mice. Human IL33 and TSLP were exclusively detectable in homozygous B-hIL33/hTSLP/hTSLPR mice but not in wild type mice. ND: not detectable.
Strain specific TSLPR expression analysis in homozygous B-hIL33/hTSLP/hTSLPR mice by flow cytometry. Bone marrow was collected from wild type C57BL/6 mice (+/+) and homozygous B-hIL33/hTSLP/hTSLPR mice (H/H), Dendritic cells were induced from bone marrow cells in culture and analyzed by flow cytometry with species-specific anti-TSLPR antibody. Mouse TSLPR was only detectable in wild type C57BL/6 mice. Human TSLPR was only detectable in homozygous B-hIL33/hTSLP/hTSLPR mice but not in wild type mice.
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