B-hIL4/hIL4RA/hIL13 mice
| Strain Name |
C57BL/6-Il4tm2(IL4)Bcgen Il4ratm1(IL4RA)Bcgen Il13tm1(IL13)Bcgen Il13ra1tm1(IL13RA1)Bcgen /Bcgen
|
Common Name | B-hIL4/hIL4RA/hIL13 mice |
| Background | C57BL/6 | Catalog number | 131037 |
|
Related Genes |
IL4: interleukin 4; IL4RA: interleukin 4 receptor, IL4R; Il13:interleukin 13 | ||
|
NCBI Gene ID |
16189,16190,16163 | ||
mRNA expression analysis
Strain specific analysis of IL4, IL4RA and IL13 gene expression in wild-type and B-hIL4/hIL4RA/hIL13 mice by RT-PCR. Mouse Il4, Il4ra and Il13 mRNA was detectable in thymocytes of wild-type mice (+/+) but not in homozygous B-hIL4/hIL4RA/hIL13 mice (H/H). Human IL4, IL4RA and IL13 mRNA was detectable only in homozygous B-hIL4/hIL4RA/hIL13 mice (H/H) but not in wild-type mice.
Strain specific IL4 expression analysis in homozygous B-hIL4/hIL4RA/hIL13 mice by ELISA.
Serum were collected from wild-type mice (+/+) and homozygous B-hIL4/hIL4RA/hIL13 mice (H/H) stimulated with anti-CD3ε in vivo, and analyzed by ELISA with species-specific IL4 ELISA kit. Mouse IL4 was detectable in wild-type mice. Human IL4 was exclusively detectable in homozygous B-hIL4/hIL4RA/hIL13 mice but not in wild-type mice.
Strain specific IL13 expression analysis in homozygous B-hIL4/hIL4RA/hIL13 mice by ELISA.
CD4+ T cells were collected from wild-type mice (+/+) and homozygous B-hIL4/hIL4RA/hIL13 mice (H/H), and then induction of CD4 + T cells differentiation into Th2 cells, supernatants from Th2 cells culture was analyzed by ELISA with species-specific IL13 ELISA kit. Mouse IL13 was detectable in wild-type mice. Human IL13 was exclusively detectable in homozygous B-hIL4/hIL4RA/hIL13 mice but not in wild-type mice.
Strain specific IL4RA expression analysis in homozygous B-hIL4/hIL4RA/hIL13 mice by flow cytometry. Splenocytes were collected from wild-type mice and homozygous B-hIL4/hIL4RA/hIL13 mice (H/H), and analyzed by flow cytometry with species-specific anti-IL4RA antibody. Mouse IL4RA was detectable in wild-type mice. Human IL4RA was exclusively detectable in homozygous B-hIL4/hIL4RA/hIL13 mice but not in wild-type mice.
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