Strain specific LGALS3 expression analysis in wild-type C57BL/6 mice and homozygous humanized B-hLGALS3 mice by ELISA. Plasma was collected from wild-type C57BL/6 mice (+/+) (n=3, 7-week-old) and homozygous B-hLGALS3 mice (H/H) (n=3, 7-week-old). Protein expression level of LGALS3 was analyzed by ELISA. Mouse LGALS3 was detectable in wild-type mice and homozygous B-hLGALS3 mice, as the kit is cross-recognize both human and mouse LGALS3. Human LGALS3 was exclusively detectable in homozygous B-hLGALS3 mice.

Strain specific analysis of LGALS3 mRNA expression in wild-type C57BL/6 mice and B-hLGALS3 mice by RT-PCR. Lung RNA were isolated from wild-type C57BL/6 mice (+/+) and homozygous B-hLGALS3 mice (H/H), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse or human LGALS3 primers. Mouse Lgals3 mRNA was detectable only in wild-type C57BL/6 mice. Human LGALS3 mRNA was detectable only in homozygous B-hLGALS3 mice but not in wild-type mice.