Western blot analysis of ADAM17 protein expression in homozygous B-hADAM17 mice. Various tissue lysates were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hADAM17 mice (H/H), and then analyzed by western blot with anti-ADAM17 antibody. 3 μg total proteins were loaded for simple western analysis. Data analysed by JESS Automated Western Blotting. ADAM17 was detected in spleen, lung, and kidney. The antibody cross recognizes human and mouse ADAM17. Black arrows: mature ADAM17 (mADAM17) and its proform (pADAM17).

Strain specific analysis of ADAM17 mRNA expression in wild-type C57BL/6 mice and B-hADAM17 mice by RT-PCR. Lung RNA were isolated from wild-type C57BL/6 mice (+/+) and homozygous B-hADAM17 mice (H/H), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse or human ADAM17 primers. Mouse Adam17 mRNA was detectable only in wild-type C57BL/6 mice. Human ADAM17 mRNA was detectable only in homozygous B-hADAM17 mice but not in wild-type mice.