| Strain Name |
|
Stock No. | 110134 |
| Common name |
B-Ucp1-iCre mice |
Gene symbol and name | Ucp1(uncoupling protein 1), Ucp; Slc25a7; AL385626 |
| Strain of origin |
C57BL/6 |
Chromosome | 8 |
| Coat color | Black | Dietary information | Growth and reproduction diet for experimental mice |
|
NCBI Gene ID |
22227 | ||
Application
- Function research of genes
- This Ucp1iCre model is an efficient tool to study various gene functions when crossed with mice with different loxP site-flanked genes of interest, especially in studies of the balance of ATP synthesis and thermogenesis in the mitochondria of brown adipose.
Expression
Expressed gene
iCre, improved cre recombinase, bacteriophage P1
Site of expression
the brown adipose tissue, adrenal adult, mammary gland adult
Gene editing strategy
A An IRES-iCre sequence cassette was placed between the coding sequence of exon 6 and 3’UTR of the Ucp1 gene using C57BL/6 embryonic stem cells. Neo cassette flanked by frt sites was inserted at least 300 bp downstream of 3’UTR. This strain was maintained on a C57BL/6 genetic background.
Phenotype Analysis
(B and C) In the virgin stage of the Ucp1-Cre-ROSAmTmG mice, the interscapular BAT was stained for mammary markers KRT14 and KRT8. (DeG) During lactation, Ucp1-GFP cells contain mammary basal myoepithelial markers KRT14 and KRT5 in the dorsal region of the anterior mammary gland (D and E) and the posterior mammary gland (F and G).
(source: Li L et al.,2017)
reference
1. Dlaskova A; Clarke KJ; Porter RK. 2010. The role of UCP 1 in
production of reactive oxygen species by mitochondria isolated from brown
adipose tissue. Biochim Biophys Acta 1797(8):1470-6. [PubMed: 20416274]
2. Fedorenko A; Lishko PV; Kirichok Y. 2012. Mechanism of Fatty-Acid-Dependent
UCP1 Uncoupling in Brown Fat Mitochondria. Cell 151(2):400-13. [PubMed:
23063128]
3. Galmozzi A; Sonne SB; Altshuler-Keylin S; Hasegawa Y; Shinoda
K; Luijten IH; Chang JW; Sharp LZ; Cravatt BF; Saez E; Kajimura S. 2014.
ThermoMouse: an in vivo model to identify modulators of UCP1 expression in
brown adipose tissue. Cell Rep 9(5):1584-93. [PubMed: 25466254]
4. Golozoubova V; Cannon B; Nedergaard J. 2006. UCP1 is
essential for adaptive adrenergic nonshivering thermogenesis. Am J Physiol Endocrinol
Metab 291(2):E350-7. [PubMed: 16595854]
5. Inokuma K; Ogura-Okamatsu Y; Toda C; Kimura K; Yamashita H;
Saito M. 2005. Uncoupling protein 1 is necessary for norepinephrine-induced
glucose utilization in brown adipose tissue. Diabetes 54(5):1385-91. [PubMed:
15855324]
6. Oelkrug R; Kutschke M; Meyer CW; Heldmaier G; Jastroch M.
2010. Uncoupling protein 1 decreases superoxide production in brown adipose
tissue mitochondria. J Biol Chem 285(29):21961-8. [PubMed: 20466728]
7. Shabalina IG; Backlund EC; Bar-Tana J; Cannon B; Nedergaard
J. 2008. Within brown-fat cells, UCP1-mediated fatty acid-induced uncoupling is
independent of fatty acid metabolism. Biochim Biophys Acta 1777(7-8):642-50.
[PubMed: 18489899]
8. Li L, Li B, Li
M, Niu C, Wang G, Li T, Król E, Jin W, Speakman JR. Brown adipocytes can
display a mammary basal myoepithelial cell phenotype in vivo. Mol Metab. 2017
Oct;6(10):1198-1211. [PMID: 29031720]
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